The human elongation factor 1 alpha (EF-1α) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter. & Jaenisch, R. At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation. S.G., H.K. You can also search for this author in

These cells accomplish such tasks through two crucial functions: first, they present small pieces of an infecting pathogen to other immune cells so those cells can learn to recognize it; and second, they instruct other cells to respond in ways that will eliminate the culprit pathogen.

Synthetic recording and in situ readout of lineage information in single cells. ), P50 HG006193 and R01 HD078679 (A.M.), F32 GM116331 (M.J.) and F32 GM125247 (J.J.Q. Spanjaard, B. et al. DNA repair profiling reveals nonrandom outcomes at Cas9-mediated breaks. For instance, a complete understanding of the regulatory network that controls immune responses can help lay the groundwork for more precise interventions, including drugs and vaccines. and M.J. provided vectors, experimental protocols and advice. The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.

and N.Y. provided phylogenetic reconstruction strategies.Scatter plots of indel proportions from dissected bulk tissue of an E9.5 embryo. Engineering synthetic signaling pathways with programmable dCas9-based chimeric receptors. Ibarra-Soria, X. et al. You can also search for this author in

Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars.

M.M.C., Z.D.S., A.M. and J.S.W. Tanay, A. You can also search for this author in

Data for financial year ending 31 March 2019. “We now have a detailed view of the circuitry that controls this critical process, providing a deeper understanding of immune biology that could inspire novel ways to treat disease and design better vaccines." Naive and primed pluripotent states. Wang, G., Moffitt, J. R. & Zhuang, X. Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy. Wang, Z. Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. Fincher, C. T., Wurtzel, O., de Hoog, T., Kravarik, K. M. & Reddien, P. W. Cell type transcriptome atlas for the planarian Plass, M. et al. Butler, A., Hoffman, P., Smibert, P., Papalexi, E. & Satija, R. Integrating single-cell transcriptomic data across different conditions, technologies, and species. The new research, which appears in the September 3 advance online edition of the journal "Our findings address a fundamental question in human biology: how do immune cells recognize various pathogens and use that information to mount distinct responses," said senior author Nir Hacohen, of the Massachusetts General Hospital (MGH) Center for Immunology and Inflammatory Diseases, an assistant professor at Harvard Medical School and a senior associate member at the Broad Institute.

Kwon, G. S., Viotti, M. & Hadjantonakis, A.-K. “We couldn’t have done this work without the efforts of our collaborators in the Broad’s RNAi Platform and the tools developed by The RNAi Consortium.” The researchers also used a single-molecule technology that enabled them to generate sensitive readouts of gene activity. But instead of tiny transistors, the internal circuitry of mammalian cells is made up of vast networks of genes and their corresponding proteins. Status.